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In recent years, with the extensive application of molecular biology techniques in the development of biological systems and other fields, there have been increasing reports on the application of PCR-based molecular biology techniques in the detection and identification of plant pathogens.
In the research and application of phytopathogenic fungi, the phylogenetic study of the predatory nematode fungus Arthrobacter spp. was carried out by PCR-RFLP method, which basically reflected the genetic relationship among different species in the genus, and proved the morphology of the Arthrobacter spp. from the molecular level. The rationality of the classification, while raising the doubts about the expansion of the genus sign; PCR amplification of the ITS region of the ribosomal genes of cotton blight bacteria, Phytophthora capsici, and the product was cloned and sequence comparison; Cooke et al. The ITS sequences of 16 species of Phytophthora ribosomes showed that there were abundant interspecies variation among different species of Phytophthora, while the sequences of different strains of the same species were highly conserved. According to the sequence analysis of ITS, the phylogenetic trends of Phytophthora oomycetes were proposed. .
In a word, the rapid diagnosis instrument for ribosomal gene ITS region has become more and more widely used in the diagnosis of various plant diseases. Based on the sequence differences of rDNAITS, various plant pathogens have been identified, providing reliable and early diagnosis of plant diseases. The basis for the future classification and molecular epidemiological investigation and other more in-depth research work laid the foundation for the prevention and control of plant diseases is also of great significance, but also to further identify the source of crop disease resistance, auxiliary crop disease resistance breeding.
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The morphology of the plant's germs is complex, and a small number of pathogens are affected by conditions such as the environment. In the past 20 years, modern molecular biology technology has made a breakthrough in the study of plant pathogenic bacteria. Some species with unclear taxonomic status and unclear genetic relationship have been validated through this technology, providing the classification, identification and diagnosis of different pathogens. A richer and more reliable means. The plant disease rapid diagnosis instrument can quickly conduct effective measurement and research on related pathogens and provide help for prevention and control.