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I. Microprocessor Control System The microprocessor control system is an operating system that maintains the steady state of temperature, humidity, and CO2 concentration in the incubator. The use of microprocessor control systems and other various function accessories (such as high and low temperature automatic adjustment and alarm devices, CO2 alarm devices, password protection settings, etc.) makes the operation and control of CO2 incubators very easy. Such as: LEEC's PID microprocessor touch screen control system, which can strictly control the concentration of gas and its loss to a very low level, in order to ensure a constant culture environment, and to ensure that the temperature inside the box during the long-term cultivation process is accurate, and LCD display, graphical process monitoring, intervention event recording, etc. In addition, the alarm system is also indispensable. It allows you to know the incubator's situation in a timely manner and react to it, thus minimizing the loss and ensuring the continuity of the experiment. Some incubators have an audible/light alarm device. When the temperature changes by ±0.5°C, or when the CO2 concentration changes by ±5%, it will automatically alarm; some have a CO2 concentration alarm display function; some have a low-voltage, power-off alarm function. These devices are designed to facilitate the user and reduce the tedious and experimental process.
Second, heating methods Air-jacketed heating and water jacket heating, both heating systems are accurate and reliable, and they all have their own advantages and disadvantages. Water jacket heating is to maintain a constant temperature through a separate water jacket surrounding the inside of the box, its advantages: water is a good insulation material, when the power is off, the water jacket system can To maintain the accuracy and stability of the temperature in the incubator for a long time, it is beneficial to the user who is not stable in the experimental environment (such as useful power restrictions or frequent blackouts). Air-jacketed heating directly heats the inner box through heaters in the air jacket of the box, which is also called six-sided direct heating. Compared with the water jacket type, the air-jacketed type has the characteristics of quick heating and quicker recovery than the water jacketed incubator, which is particularly advantageous for short-term culture and the need for frequent switching of the door and setting of the sample. In addition, for the user, the air-jacketed design is simpler than the water-jacketed type (the water-jacketed type requires watering, emptying and cleaning of the water tank, and often monitors the operation of the water tank, as well as potential pollution hazards).
Second, carbon dioxide concentration control infrared sensor (IR) or thermal conductivity sensor (TC) measurements. Both sensors have their own advantages and disadvantages. The working principle of the thermal conductivity sensor monitoring CO2 concentration is based on the continuous measurement of the thermal conductivity of the cavity air, the low thermal conductivity of the input CO2 gas will cause the thermal conductivity of the air in the cavity to change, which will produce a direct relationship with the CO2 concentration. A proportional electrical signal. One of the disadvantages of the TC control system is that changes in the temperature and relative humidity inside the box affect the accuracy of the sensor. When the door is opened frequently, not only the CO2 concentration, temperature, and relative humidity will also fluctuate greatly, thus affecting the accuracy of the TC sensor. When precise culture conditions and frequent opening of the incubator door are required, this control system becomes less applicable. Infrared Sensor (IR) It uses an optical sensor to detect CO2 levels. The IR system includes an infrared emitter and a sensor. When CO2 in the box absorbs some of the infrared rays emitted by the emitter, the sensor can detect the reduction of infrared rays. The amount of infrared rays absorbed corresponds exactly to the level of CO2 in the box. Therefore, the concentration of CO2 in the box can be found. Since the IR system determines the CO2 concentration in the box through infrared reduction, and the particulate matter in the box can reflect or partly absorb infrared rays, the IR system is more sensitive to the amount of particulate matter in the box. The IR sensor uses a HEPA high efficiency air filter at the air inlet. The incubator is more suitable.
Third, the relative humidity inside the box is a very important but often overlooked factor for the cultivation work. Maintaining a sufficient level of humidity and having a sufficiently fast rate of humidity recovery (eg after opening and closing the door) ensures that the culture will not fail due to excessive drying. Most current CO2 incubators produce moisture through the evaporation of the humidifying pan (which produces a relative humidity level of about 95%, but the humidity is slow to recover after opening the door). Try to choose an incubator with a large humidity evaporation area, because the larger the humidity evaporation area, the easier it is to reach the maximum relative saturation humidity and the shorter the humidity recovery time behind the door.
V. Contaminant Control Pollution is a major factor in the failure of cell culture. Manufacturers of carbon dioxide incubators have designed many different devices to reduce and prevent the occurrence of pollution. The main approach is to minimize the number of microorganisms that can grow. Zones and surfaces, combined with automatic decontamination devices to effectively prevent contamination. For example, in view of the fact that the CO2 incubator is sometimes accompanied by mold growth during its use, some companies have developed and designed a CO2 incubator with UV disinfection to protect the incubator from contamination and ensure biological cleanliness in the instrument housing. A company-designed HEPA high-efficiency filter can filter the air in the incubator and can filter out 99.97% of the 0.3um or more particles.
There are two basic requirements for users of carbon dioxide incubators. First, the carbon dioxide incubator is required to provide the most accurate and stable control of temperature, carbon dioxide concentration, and humidity in order to facilitate the progress of its research work; the second is to require that the carbon dioxide incubator be able to Effective prevention of microbial contamination in the incubator and regular elimination of contamination to protect research results and prevent sample loss.